Biochemistry

Volhard-Harvey test: Estimation of chloride in urine

Chloride concentration of urine is dependent upon the amount of chloride present in the ingested food.  Volhard-Harvey Method: PRINCIPLE: The chloride in the urine is precipitated as silver chloride with a measured excess of silver nitrate The excess of silver nitrate is treated With ammonium. presence of Ferric Ammonium Sulphate as an indicator. 1 Concentrated …

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Benedict’s Test: Estimation Glucose in urine

The amount of glucose present in the urine of a normal subject is about 100mg/day, which is not detectable by the commonly employed qualitative methods. However, in some persons glucose may appear in the urine as in benign glucosuria which is not uncommon. METHOD: Benedict’s Method: PRINCIPLE: Glucose or reducing substances in the urine reduces …

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Estimation serum Creatinine By Folin’s Method (Jaffe’s Reaction)

Creatinine is an anhydride of creatine. It is formed in the muscle from creatine phosphate by an irreversible, non-enzymatic dehydration and loss of phosphate. Serum creatinine (a blood measurement) is an important indicator of renal health because it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys METHOD: Folin’s …

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Satyanarayana Biochemistry pdf Review & Download Free:

Biochemistry is that branched of medical science which deal with the chemical processes within or related to living system. Human Biochemistry deal with the chemical process in  the human being.  It is one of the basic subject of medical science. Because without study biochemistry, you can understand pharmacology and histo-pathology. Fortunately there are many great …

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