The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Leishman’s Stain: 1.5 gm powder of Leishman’s stain …
Preparation Blood Smear (blood film) & Staining: Read More »
Apparatus: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid): Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2504): 2.5 gm, which breaks RBCs and prevents their rouleaux formation. Mercuric chloride (HgClz): 0.25 gm act as preservative (antibacterial and …
Haemocytometer is an apparatus used to count various blood cells (RBC , WBC and a easinophll and platelets). It consists of RBC and WBC pipette (Thoma diluting pipettes) thick slide (Neubauer’s chamber). REAGENTS: Hayem’s fluid for RBC counting and its composition: NaCl 0.5 g for isotonicity. Na2SO4- 2.5 g Breaks R.B.Cs and prevents their Rouleaux formation. …
Study Haemocytometer Principle & How To Collect Blood Sample: Read More »
GLANDS: A gland may be defined as a single cell or a group of cells, which are specialized for secretion(useful products;, or excretion (useless products). All gland cells produce and expel, to the extracellular compartment, produced that are not used by the cell itself but are of importance to other parts of the organism. CLASSIFICATION: Glands …