Monthly Archive:: February 2017

langman embryology

Estimation of hemoglobin by Sahli’s method:

Apparatus: Hemoglobinometer, pricking needle (blood lancet) and spirit swab. Sahli’s method: Hemoglobinometer: It is a box which contains: Stirrer: Simple glass rod used to mix the blood with acid or distilled water. Hemoglobin pipette: it is a simple pipette having capillary tube …
Erythrocyte Sedimentation Rate

Erythrocyte Sedimentation Rate (ESR):

DEFINITION OF Erythrocyte Sedimentation Rate: ESR is a measure of settling down of RBCs in an anticoagulated specimen of whole blood during a specified period of time. APPARATUS WST = Westergren’s tube WTB = Wintrobe’s tube Westergren’s tube (pipette) with stand, Wintrobe’s …
clinical examination of precordium

Clinical Examination Of Precordium:

THEORY. Precordium is the portion of the anterior aspect of the chest wall which overlies the heart. Its examination is carried out under following headings: Palpation Inspection percussion. Auscultation  Inspection Clothing of the patient should be removed upto waist. Look for: Veins …
Blood pressure

blood pressure measurement:

PRINCIPLE : The principle of the experiment involves the balancing of air pressure against the pressure of blood in brachial artery, the air pressure being estimated by a mercury  or air (aneroid) manometer . A cloth-covered rubber bag is wrapped around upper arm, …
Leukocyte count

Differential leukocyte count:

APPARATUS (Reagents and equipments): 4-5 clean glass slides, Leishman’s stain, blood lancet, dropper, glass rod, compound microscope, cedar wood oil, buffered water and staining tray. PRINCIPLE: A blood film appropriately prepared and stained is seen under the microscope in oil immersion. Different …
preparation of Blood Smear

Preparation Blood Smear (blood film) & Staining:

The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, …
RBC count

Determination Red Blood Cell count:

Apparatus: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid): Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2504): 2.5 gm, which breaks RBCs and prevents …