Month: February 2017

Estimation of hemoglobin by Sahli’s method:

Apparatus: Hemoglobinometer, pricking needle (blood lancet) and spirit swab. Sahli’s method: Hemoglobinometer: It is a box which contains: Stirrer: Simple glass rod used to mix the blood with acid or distilled water. Hemoglobin pipette: it is a simple pipette having capillary tube in it. This is connected to the teat (mouth piece) through a rubber …

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Erythrocyte Sedimentation Rate (ESR):

DEFINITION OF Erythrocyte Sedimentation Rate: ESR is a measure of settling down of RBCs in an anticoagulated specimen of whole blood during a specified period of time. APPARATUS WST = Westergren’s tube WTB = Wintrobe’s tube Westergren’s tube (pipette) with stand, Wintrobe’s tube with stand, long nozzled capillary pipette (Pasture pipette), anticoagulants 3.8% sodium citrate …

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Preparation Blood Smear (blood film) & Staining:

The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Leishman’s Stain: 1.5 gm powder of Leishman’s stain …

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Determination Red Blood Cell count:

Apparatus: Neubauer’s chamber (thick slide), RBC diluting fluid, microscope, coverslip, pricking needle (blood lancet) and spirit swab. RBC Diluting Fluid (Hayem’s Fluid): Sodium chloride (NaCl): 0.5 gm, to maintain isotonicity of fluid. Sodium sulphate (Na2504): 2.5 gm, which breaks RBCs and prevents their rouleaux formation. Mercuric chloride (HgClz): 0.25 gm act as preservative (antibacterial and …

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Study Haemocytometer Principle & How To Collect Blood Sample:

Haemocytometer is an apparatus used to count various blood cells (RBC , WBC and a easinophll and platelets). It consists of RBC and WBC pipette (Thoma diluting pipettes) thick slide (Neubauer’s chamber). REAGENTS: Hayem’s fluid for RBC counting and its composition: NaCl 0.5 g for isotonicity. Na2SO4- 2.5 g Breaks R.B.Cs and prevents their Rouleaux formation. …

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